The present invention relates to a method for separating larvae into a pulp fraction and a liquid fraction, including the steps of introducing living larvae into a grinding apparatus whist adding water, grinding the larvae by means of counter-rotating screws and separating the ground biomass of larvae into a pulp and liquid fraction. In particular, the invention is applicable to the larvae of the black soldier fly and produces a chitin-rich pulp and a fat-and-protein-rich liquid fraction.

The disclosure describes methods for the purification of protein-enriched extracts to provide concentrates and isolates and methods for incorporation of such materials into products. The purification methods are adapted for removal of, e.g., chlorophyll and may thus provide lightening the color of the protein-enriched extracts. The methods generally include treatment with peracetic acid or hydrogen peroxide and filtrations. A protein composition in the form of a concentrate or isolate is provided, the protein composition including RuBisCO, F2 fraction proteins, or combination thereof extracted from a plant material.

The present disclosure relates, according to some embodiments, to methods and systems for processing a high-concentration protein product from a microcrop (e.g., aquatic species, Lemna) and compositions thereof. According to some embodiments, the present disclosure relates to a method of processing a biomass comprising a microcrop (e.g., Lemna), where the method may include: blanching a first portion of the biomass in a blanching solution to form a wet protein concentrate; separating the first wet protein concentrate from a separated solution (e.g., using a screw press, using a vibratory screen); and drying the first wet protein concentrate to form at least one of a first protein concentrate flake and a first protein concentrate granule. In some embodiments at least one of the first protein concentrate flake and the first protein concentrate granule may comprise at least 45% protein and a Protein Digestibility Corrected Amino Acid Score (PDCASS) value of at least 0.88. In some embodiments, the present disclosure relates to protein products and compositions derived from a microcrop (e.g., derived from Lemna).

The present disclosure relates, according to some embodiments, to methods and systems for processing a high-concentration protein product from a microcrop (e.g., aquatic species, Lemna) and compositions thereof. According to some embodiments, the present disclosure relates to a method of processing a biomass comprising a microcrop (e.g., Lemna), where the method may include: blanching a first portion of the biomass in a blanching solution to form a wet protein concentrate; separating the first wet protein concentrate from a separated solution (e.g., using a screw press, using a vibratory screen); and drying the first wet protein concentrate to form at least one of a first protein concentrate flake and a first protein concentrate granule. In some embodiments at least one of the first protein concentrate flake and the first protein concentrate granule may comprise at least 45% protein and a Protein Digestibility Corrected Amino Acid Score (PDCASS) value of at least 0.88. In some embodiments, the present disclosure relates to protein products and compositions derived from a microcrop (e.g., derived from Lemna).

The invention relates to a process for the production of a protein mass from insect larvae, comprising the steps of, cleaning insect larvae, applying pulsed electric fields to the cleaned insect larvae and comminuting the insect larvae to produce an insect mass; and subsequently fractionating the insect mass into a solid and at least one liquid phase with subsequent drying of the solid.

The invention relates to a process for the production of a protein mass from insect larvae, comprising the steps of, cleaning insect larvae, applying pulsed electric fields to the cleaned insect larvae and comminuting the insect larvae to produce an insect mass; and subsequently fractionating the insect mass into a solid and at least one liquid phase with subsequent drying of the solid.

The present invention relates to a method for converting insects into a nutrient stream, such as a puree, an enzymatically hydrolysed puree, a fat fraction and a protein fraction. The invention further relates to the (hydrolysed) puree and to the fractions obtainable by said methods and to their use as a food or feed product or as an ingredient therefore. The invention also relates to a method for converting insect larvae into a composition with anti-oxidant properties. Thus, the invention also relates to a method for converting insect larvae into an antioxidant composition. In addition, the invention relates to a composition with anti-oxidant properties obtainable by said method and to the use of the composition as a health promoting food ingredient or as a feed ingredient.

The presently disclosed subject matter provides methods of treating animal proteins and pet food products including the same. Methods include treating animal proteins with one or more base compounds to increase the pH thereof to provide for increased preservation and the reduction of spoilage of the animal protein.

A method of dehydrating and rehydrating mycelium includes growing fungal cells in a growth media such that the fungal cells produce a mycelium mass having a protein content of greater than 40 wt % of a dry mass of the mycelium mass. The method includes separating the mycelium mass from the growth media, compacting the mycelium mass, and dehydrating the compacted mycelium mass to produce a dehydrated mycelium mass having a moisture content in a range of 5 wt % to 60 wt % and a first hardness in a range of 0.007 kgf/mm.sup.2 to 0.018 kgf/mm.sup.2. The method includes rehydrating the dehydrated mycelium mass to form a rehydrated mycelium mass having a moisture content of greater than 60 wt % and a second hardness in a range of 0.00035 kgf/mm.sup.2 to 0.007 kgf/mm.sup.2.

This disclosure provides novel silk fibroin protein based additives for foods or beverage products, and foodstuffs mixed or coated with pure silk fibroin-based proteins or protein fragments, and methods of making thereof.