The present disclosure relates to the production of steviol glycosides rebaudioside J and rebaudioside N through the use of rebaudioside A as a substrate and a biosynthetic pathway involving various 1,2 RhaT-rhamnosyltransferases.

The present invention is directed to compositions comprising inter alia, an oligosaccharide, a monk fruit-derived material, a masking agent, and a soluble fiber, and use of same, such as a sugar substitute.

The present invention relates, at least in part, to the production of steviol glycoside rebaudioside I through the use of variant UGT enzymes having activity to transfer a glucosyl group from UDP-glucose to rebaudioside A to produce rebaudioside I.

Disclosed herein is an all-natural sugar substitute composition-of-matter, which includes an indigestible soluble fiber ingredient, a natural high-potency sweetening ingredient, a flavor-balancing ingredient, a taste-balancing ingredient, and a tiger nut product ingredient, which can replace sugar in any food, cosmetic or pharmaceutical product.

An aspect of the present invention provides a method for producing a rebaudioside D-containing aqueous composition comprising rebaudioside D at a concentration of 500 ppm by weight or more, the method comprising a step of stirring for dispersing a steviol glycoside component containing rebaudioside D and water in a temperature range of 40° C. to 100° C. under high shear at a tip speed (peripheral speed) of a rotor of 15 m/s or more, wherein the steviol glycoside component comprises more than 50% by weight of rebaudioside D based on a total weight of the steviol glycoside component.

Natural sweetener compositions are disclosed, which generally include various combinations of steviol glycosides extracted from a Stevia rebaudiana plant; Siraitia grosvenorii (Monk Fruit) plant extract (including mogrosides extracted from such plant species); and an amino acid component consisting of free glycine. The natural sweetener compositions may optionally further include gymnemic acid (such as an extract of a Gymnemic sylvestre herb that includes 25%-75% gymnemic acid). The natural sweetener compositions may consist essentially of those two, three, or four components—or may be included in beverages, beverage powders, and foods.

The present invention provides a plant body containing a compound represented by Formula (1), or a salt or a hydrate thereof:

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[in the formula: (i) R.sub.1 represents Xyl(1-2)Glc1- and R.sub.2 represents Glc(1-2)[Glc(1-3)]Glc1- or (ii) R.sub.1 represents Glc(1-2)[Glc(1-3)]Glc1- and R.sub.2 represents Xyl(1-2)[Glc(1-3)]Glc1- (where Glc stands for glucose and Xyl stands for xylose)].

Beverage syrups and finished beverages comprising a sweetener blend of a steviol glycoside mixture comprising rebaudioside M and certain synthetic sweeteners in particular amounts that provide improved sensory profiles are described herein. Also provided are methods of preparing beverage syrups and finished beverages.

A steviol glycoside composition having a total content of specific nine types of steviol glycosides is equal to or more than 60% by weight and less than 95% by weight and the total content of other steviol glycosides and stevia-derived components is 5 to 40% by weight each relative to the total solid content weight of composition, weight-based ratios of rebaudioside A and rebaudioside D relative to the total content of the nine steviol glycosides are 35 to 75% and 5 to 30% by weight, respectively, and the composition has at least one of: methanol content is 0.10% by weight or less relative to the total content of the nine steviol glycosides; polyphenol content is 2.0% by weight or less relative to the total content of the nine steviol glycosides; and nitrogen amount is 0.40% by weight or less relative to the total amount of the nine steviol glycosides.

The present invention provides a novel steviol glycoside containing xylose. According to the present invention, there is provided a compound represented by formula (1), or a salt or hydrate thereof. (In formula, (i) R.sub.1 represents Xyl(1-2)Glc1- and R.sub.2 represents Glc(1-2)[Glc(1-3)]Glc1-; or (ii) R.sub.1 represents Glc(1-2)[Glc(1-3)]Glc1- and R.sub.2 represents Xyl(1-2)[Glc(1-3)]Glc1-, where Glc represents glucose and Xyl represents xylose.)

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