Methods for recombinant production of steviol glycoside and compositions containing steviol glycosides are provided by this invention.

The invention relates to a glucose-containing sweetening mixture, comprising or consisting of glucose and at least one glucose-free sweetener, wherein the sweetening mixture has a sweetness between 3.0 and 5.0, in particular between 3.5 and 4.5. The invention further relates to a food product containing this sweetening mixture.

The disclosure relates to the field of food ingredients, specifically to sweeteners, more specifically to steviol glycosides and improved isolation thereof.

Various embodiments of the present technology provide compositions and methods for a sweetener composition comprising stevia leaf extract, stevia glycosides, and/or Luo Han Guo fruit extract. The sweetener composition provides enhanced taste and/or flavor properties as compared to other non-caloric or low-caloric sweeteners. The sweetener composition has a reduced aftertaste relative to stevia leaf extract and/or purified steviol glycoside. The sweetener composition may be formulated into any edible sweetened substances.

A traditional Chinese medicine composition includes 60%-80% by weight of Eucommia ulmoides extract, 15%-30% by weight of Rhizoma Drynariae extract and 5%-10% by weight of Semen Cuscutae extract. A method of preparing the traditional Chinese medicine composition is also provided. Uses of the traditional Chinese medicine composition in the prevention and/or treatment of osteoporosis are also provided.

Methods of preparing highly purified steviol glycosides. The methods include utilizing enzyme preparations and recombinant microorganisms for converting various starting compositions to target steviol glycosides. The highly purified steviol glycosides are useful as non-caloric sweetener, flavor enhancer, sweetness enhancer, and foaming suppressor in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.

The disclosure relates to enzymes, such as cucurbitadienol synthase (CDS), UDP-glycosyltransferase (UGT), C11 hydroxylase, epoxide hydrolase (EPH), squalene epoxidase (SQE), and/or cytochrome P450 reductase enzymes, recombinant host cells expressing the enzymes, and methods of producing mogrol precursors, mogrol, and/or mogrosides using such recombinant cells.

The invention describes compositions that include a stevia sweetener and a salt of a steviol glycoside, wherein the concentration of the components provide an improved taste profile where bitterness, after taste and/or lingering of the stevia sweetener is decreased or eliminated.

The present invention provides a composition which contains a steviol and/or steviol glycoside, and is for inhibiting the accumulation of fat, promoting lipolysis, reducing blood glucose level, enhancing the intake of glucose, improving the metabolism of glucose, or treating alcoholic liver disease or non-alcoholic fatty liver disease.

The present invention pertains to a novel complex of rebaudioside D having increased water-solubility, and a novel method for increasing the solubility of rebaudioside D in water. The present invention provides a complex containing Reb. D and one or more compounds selected from sugars as well as water-soluble vitamins and salts thereof, wherein the water-solubility of Reb. D at a water temperature of 25° C. is 75 mg/100 g-H.sub.2O or higher.