A three-dimensional electrospun nanofiber scaffold for use in repairing a defect in a tissue substrate is provided. The three-dimensional electrospun nanofiber scaffold includes a first layer formed by a first plurality of electrospun polymeric fibers and a second layer formed by a second plurality of electrospun polymeric fibers. The second layer is coupled to the first layer using a coupling process and includes a plurality of varying densities formed by the second plurality of electrospun polymeric fibers. The first and second layers are configured to degrade via hydrolysis after at least one of a predetermined time or an environmental condition. The three-dimensional electrospun nanofiber scaffold is configured to be applied to the tissue substrate containing the defect.

This document provides methods and materials for treating nerve injuries and/or neurological disorders. For example, compositions including an amnion tissue preparation and/or a stem cell preparation as well as methods for using such compositions to treat a nerve injuries and/or neurological disorders are provided.

Non-woven graft materials for use in specialized surgical procedures such as neurosurgical procedures, methods for making the non-woven graft materials, and methods for repairing tissue such as neurological tissue using the non-woven graft materials are disclosed. More particularly, disclosed are non-woven graft materials including at least two distinct fiber compositions composed of different polymeric materials, methods for making the non-woven graft materials and methods for repairing tissue in an individual in need thereof using the non-woven graft materials.

The invention generally relates to a method of regenerating a nerve fiber in a damaged neural tissue of a patient, the method comprising the steps of: administering an aqueous formulation comprising superparamagnetic particles to the damaged neural tissue in the patient; applying a magnetic field in an orientation which is parallel to the nerve fiber; using the magnetic field for aligning the superparamagnetic particles; forming one or more aligned chains of the superparamagnetic particles in the magnetic field as a scaffold to guide directional growth of regenerating nerve cells; and reconnecting damaged nerve ends in the damaged neural tissue of the patient.

Methods and techniques for forming high concentration hydrogels are disclosed herein. The presently disclosed high concentration hydrogels are formed using controlled dehydration and optional rehydration techniques, depending on desired use. The disclosed high concentration hydrogels may include agarose with or without other hydrogels or therapeutic agents, such as hyaluronic acid, present.

A composite lumen includes an extruded tube of a composite including a poly(glycerol sebacate) (PGS) matrix mixed with a PGS thermoset filler. The composite lumen also includes an overbraid structure overlying an outer surface of the extruded tube. A method of forming a composite lumen includes extruding a PGS tube of a composite including a PGS matrix mixed with a PGS thermoset filler. The method also includes applying an overbraid structure over an outer surface of the extruded tube.

The proposed invention relates to the medical biotechnologies, to the tissue-derived approaches in regenerative medicine in particular. The method proposes, in addition to conservative treatment methods, substitution therapy for skin damage with the aid of polymer matrices, which are similar to the histotypically of the organism tissues with biologically active agents such as cellular derivatives (collagens and laminins) that contribute to the structural function of the damaged area. A biodegradable wound-healing composite material based on a combination of a polymer substrate and a product of the synthesis of human epithelial cells, devoid of the cell component, is characterized by a relative simplicity of manufacture, the long duration of the storage and can make possible to avoid skin grafting, for example in cases of deep and extensive burns, trophic ulcers, etc.

The disclosure discloses a tissue-engineered nerve transplant and a preparation method thereof, and belongs to the technical fields of biomaterials and tissue engineering. By optimizing the specification of stripes, the stripes can independently induce EMSCs to differentiate to myelination cells (Schwann cells) to the maximum extent so as to obtain an EMSCs/biomaterial scaffold compound. The EMSCs/biomaterial scaffold compound can not only be used as a three-dimensional cell culture model for researching neural stem cell differentiation, nerve fiber growth and myelination molecular mechanisms in vitro, but also be used as a tissue engineering transplant for in-vivo transplantation to repair nervous system injury. In the disclosure, an EMSCs/micropatterned biomaterial film is rolled into a cylindrical multi-tunnel type nerve regeneration conduit to be used to repair sciatic nerve injury by transplantation, and results show that the disclosure can promote nerve regeneration and recovery of a lower limb motor function through injured portion transplantation, and has good clinical application prospects and research and development value.

A method for making iridium oxide nanoparticles includes dissolving an iridium salt to obtain a salt-containing solution, mixing a complexing agent with the salt-containing solution to obtain a blend solution, and adding an oxidating agent to the blend solution to obtain a product mixture. A molar ratio of a complexing compound of the complexing agent to the iridium salt is controlled in a predetermined range so as to permit the product mixture to include iridium oxide nanoparticles.

The present application provides a poly(allylguanidine) and the manufacturing process thereof. In addition, the present application further provides uses of the poly(allylguanidine), which can be applied in culturing neurons or as an implant for the affected area of a brain tumor after surgical procedure.