The present invention relates to the compositions, methods, and associated devices (1) for the cryopreservation of biological specimens that enables to freeze and thaw the entire specimen by straightforwardly protecting its three-dimensional architecture. The invention comprises compositions including reagents to protect the specimen and preserve the physiological conditions. The methods comprise fewer steps excluding the need for other solutions used in conventional methods. The methods also eliminate stressful steps for biological specimens in conventional methods including harvesting, pipetting, centrifuging, and transferring. With this invention, the entire content of the specimen can be frozen, stored long-term in a single cell culture vessel with one of the associated devices (1) described here, and then thawed while still in the device (1). The biological specimens frozen by that method render high post-thawing viability. Moreover, the invention reduces cryoprotectant-related toxic events, human errors, and contamination risk.

Methods and materials for cryopreservation of precision-cut tissue slices, including precision-cut liver slices (PCLS), where the slices are vitrified. A cryoprotective solution comprising high amounts of ethylene glycol in combination with sucrose are used and high cooling rates greater than the critical cooling rate for avoidance of ice formation are used in a method which results in vitrification of the tissue slices without toxicity and with increased viability after rewarming. Methods and materials can be used for production of PCLS which can be stored at cryoprotective temperatures for extended periods of time.

The present disclosure provides a cell patch cryopreservation solution, which includes: a cryoprotectant, a buffer, a reducing agent, a calcium channel inhibitor and an apoptosis inhibitor. The present disclosure also provides a method of cryopreserving a cell patch by using the aforementioned cell patch cryopreservation solution. The cell patch cryopreservation solution and the method of cryopreserving a cell patch provided by the present disclosure can maintain the structural integrity and the cell activity of the cell patch.

Provided herein, inter alia, are compositions and methods including T-cell cultures enriched for gdT cells, the gdT cells expressing a CAR, and related methods for generating said cells. In an aspect provided herein is a method for generating a T-cell culture enriched for gamma delta T-cells (gd T-cells or T cells). In another aspect, a method for generating a gdT-cell expressing a Chimeric Antigen Receptor (CAR) is provided. The method includes introducing a nuclei acid encoding a CAR to a gdT-cell obtained as provided herein, including embodiments thereof.

Some embodiments relate to improved cryopreservative formulations and improved methods for cryopreserving and thawing cells. These formulations facilitate high density, low volume cell cryopreservation strategies which overcome the need for washing of cells post-thaw, and therefore result in greater cell recovery and viability. Pharmaceutical compositions comprising a cell population and a cryopreservative formulation as provided herein are also provided. Methods for treating a subject having a disease or condition that benefits from transplantation of a cryopreserved and thawed cell population are also provided.

It is an object of the present invention to provide a composition for cryopreservation while reducing cell damage due to cryopreservation and to provide a cryopreservation method while reducing cell damage due to cryopreservation. A composition for cryopreservation of a cell or biological tissue, the composition comprising a nanoparticle that comprises amphiphilic molecules as a constituent, wherein the amphiphilic molecules form a monolayer or bilayer, is produced.

The disclosure relates generally to methods and compositions for cryopreservation and processing of blood for single-cell RNA-sequencing. More particularly, the disclosure relates to methods and compositions for preserving and processing whole blood to enable diagnosing and/or treating sepsis via single-cell RNA sequencing (scRNA-seq). In certain aspects, the methods and compositions disclosed herein may be employed in diagnosis and treatment of subjects having or at risk of having sepsis.

An object of the present invention is to provide an agent for promoting undifferentiation that dissolves a drug, as DMSO or the like does, but is capable of promoting undifferentiation without inducing cell differentiation when added to a medium. The agent for promoting undifferentiation of the present invention has an aprotic zwitterion represented by the following formula (1)

##STR00001##

wherein A is an anion selected from the group consisting of SO.sub.3.sup., COO.sup., OPO(H)O.sup., OPO(CH.sub.3)O.sup. and OPO(OR.sub.3)O.sup., R.sub.1 is an alkyl group having 1 to 8 carbon atoms and optionally containing one or two oxygen atoms in the molecular chain, R.sub.2 is an alkylene group having 3 to 5 carbon atoms, and R.sub.3 is hydrogen or an alkyl group optionally having a heteroatom in the molecular chain.

Compositions for solid-state storage of a biological system and methods of making and using the same are described. The compositions comprise a polymeric cryoprotectant, a saccharide cryoprotectant, an emulsifier, and at least one lubricating flow agent, and are combined with a biological system to store the biological system in a solid-state with or without cold-chain infrastructure. The stabilized biological systems are suitable for use with one or more analytical devices to provide a cold chain-free, point-of-care, and low-cost platform for diagnostics, research, biomanufacturing, and testing.

Embodiments of the present invention may provide protecting, preserving, and even repairing biological cells during in vitro processing. A uniform environment such as a protective layer with exogeneous lipids may be formed around biological cells which may interact with the plasma membranes of the cells.