A semiconductor structure includes a semiconductor substrate, an insulating layer, a conductive feature and an anisotropic conductive structure. The insulating layer is disposed above the semiconductor substrate. The conductive feature is disposed in the insulating layer, wherein a top surface of the conductive feature is adjacent to a top surface of the insulating layer. The anisotropic conductive structure is disposed on the insulating layer and the conductive feature. The anisotropic conductive structure includes a metal oxide porous layer and conductive pillars. The metal oxide porous layer has a first nano-through-hole array exposing the top surface of the conductive feature and a second nano-through-hole array exposing the top surface of the insulating layer. The conductive pillars fill the first nano-through-hole array, wherein the conductive pillars are in contact with the top surface of the conductive feature.