STEVIOL GLYCOSIDE

Abstract

Nobel Stevia Sweetening components are provided. Through the analysis of the components of the nobel Steviol Glycoside included in the stevia extract and/or crystals, not only the quality control of sweeteners, but judgment on the correctness of indication of origin or infringement of right are facilitated since the raw material of the sweetener can be identified.

Claims

1.-7. (canceled)

8. A method of manufacturing of a sweetener comprising Steviol glycoside selected from the group consisting of Steviol glycoside III (Rebaudioside I), Steviol glycoside IV (Rebaudioside H), Steviol glycoside VI (Rebaudioside K), Steviol glycoside VII (Rebaudioside K), Steviol glycoside IX (Rebaudioside N) and Steviol glycoside X (Rebaudioside O) of the formula: ##STR00015## wherein R.sub.1 and R.sub.2 represent sugar chains defined in the following table; TABLE-US-00012 No. (Steviol Glyciside) R.sub.1 R.sub.2 III (Rebaudioside I) B-glc-B-glc(3.fwdarw.1) IV (Rebaudioside H) B-glc V (Rebaudioside L) B-glc VI (Rebaudioside K) Bglc-B-glc(2.fwdarw.1) VII (Rebaudioside J) B-glc--rha(2.fwdarw.1) IX (Rebaudioside N) X (Rebaudioside O) the method comprising: purifying the Steviol glycoside such that the Steviol glycoside is separated from Rebaudioside A and formulating the sweetener with the Steviol glycoside; wherein the sweetener comprises a larger amount of the Steviol glycoside than any other Steviol glycoside.

9. The method of claim 8, wherein the Steviol glycoside is purified from Stevia Rebaudiana Bertoni.

10. The method of claim 8, further comprising crystalizing an extract obtained from the purifying step.

11. The method of claim 8, wherein the purifying comprises use of a column.

12. The method of claim 11, further comprising crystalizing an extract obtained from the purifying step.

13. The method of claim 12, wherein the crystals comprise a larger amount of the Steviol glycoside than Rebaudioside A.

14. The method of claim 12, wherein the crystals comprise a larger amount of the Steviol glycoside than any other Steviol glycoside.

15. The method of claim 8, wherein the purifying comprises adsorbing the Steviol glycoside to an adsorbant resin and eluting the Steviol glycoside from the adsorbant resin to produce an extract.

16. The method of claim 15, further comprising crystalizing the extract obtained from the purifying step.

17. The method of claim 15, wherein the extract comprises a larger amount of the Steviol glycoside than any other Steviol glycoside.

18. The method of claim 8, wherein an extract obtained from the purifying step comprises a larger amount of the Steviol glycoside than Rebaudioside A.

19. The method of claim 8, wherein the purifying comprises purifying the Steviol glycoside from a Stevia Rebaudiana Bertoni variety which contains more Rebaudioside A content than Stevioside.

20. A method of preparing a food product, the method comprising manufacturing the sweetener according to the method of claim 8, and mixing the sweetener and a food to form the food product.

21. A method of preparing a food product, the method comprising manufacturing the sweetener according to the method of claim 11, and mixing the sweetener and a food to form the food product.

22. A method of preparing a food product, the method comprising manufacturing the sweetener according to the method of claim 12, and mixing the sweetener and a food to form the food product.

23. A method of extracting Steviol glycoside selected from the group consisting of Steviol glycoside III (Rebaudioside I), Steviol glycoside IV (Rebaudioside H), Steviol glycoside VI (Rebaudioside K), Steviol glycoside VII (Rebaudioside K), Steviol glycoside IX (Rebaudioside N) and Steviol glycoside X (Rebaudioside O) from a solution of the Steviol glycoside and water or a solvent containing water, comprising extracting the solution with water or a solvent containing water to form an extract comprising the Steviol glycoside, wherein the extract comprises a higher concentration of the Steviol glycoside than the solution, and the extract comprises a larger amount of the Steviol glycoside than any other Steviol glycoside wherein the Steviol glycoside has the formula: ##STR00025## wherein R.sub.1 and R.sub.2 represent sugar chains defined in the following table TABLE-US-00013 No. (Steviol Glyciside) R.sub.1 R.sub.2 III (Rebaudioside I) B-glc-B-glc(3.fwdarw.1) IV (Rebaudioside H) B-glc V (Rebaudioside L) B-glc VI (Rebaudioside K) Bglc-B-glc(2.fwdarw.1) VII (Rebaudioside J) B-glc--rha(2.fwdarw.1) IX (Rebaudioside N) X (Rebaudioside O)

24. The method of claim 23, wherein the extracting comprises use of a column.

25. The method of claim 23, wherein the extracting comprises a lower concentration of Rebaudioside A compared to the Rebaudioside A concentration in the solution.

26. The method of claim 25, wherein the extracting comprises use of a column.

27. The method of claim 23, wherein the extracting comprises use of a resin adsorbing Steviol glycosides.

28. The method of claim 23, wherein the solution is obtained from a Stevia Rebaudiana Bertoni variety which contains more Rebaudioside A content than Stevioside.

Description

BRIEF DESCRIPTION OF DRAWINGS

[0090] FIG. 1 It shows the HPLC Analysis Chart for the extract RA-C.

[0091] FIG. 2 It shows the HPLC Analysis Chart for Crystals RA-A.

[0092] FIG. 3 It shows the HPLC Analysis Chart for Crystals RA-B.

[0093] FIG. 4 It shows the HPLC Analysis Chart for Crystals RA-C.

[0094] FIG. 5 It shows the HPLC Analysis Chart for the extract RA-A.

[0095] FIG. 6 It shows the HPLC Analysis Chart for the extract RA-B.

[0096] FIG. 7 It shows the HPLC Analysis Chart for the extract RA-C.

[0097] FIG. 8 It shows the HPLC Analysis Chart for the extract ST.

[0098] FIG. 9 It shows the HPLC Analysis Chart for Crystals ST-ST.

[0099] FIG. 10 It shows the HPLC Analysis Chart for Crystals ST-RA.