A perfusion system (100) includes a perfusion module (110) configured to circulate a perfusate. The perfusion module includes an oxygenator (112) configured to oxygenate the perfusate, the oxygenator configured to be fluidly coupled with an oxygen source and configured to receive oxygen therefrom, and a first and a second pump, each pump operably coupled with the oxygenator and configured to circulate the perfusate through the oxygenator. The system further comprises a cannister (140) and a tissue interface (150) disposed between the perfusion module and the cannister. The first and second pumps can be diaphragm pumps. The oxygen source can be in fluid communication with the perfusion module to allow flow of oxygen and pressurization of the pump diaphragms (128).

Methods and materials for cryopreservation of precision-cut tissue slices, including precision-cut liver slices (PCLS), where the slices are vitrified. A cryoprotective solution comprising high amounts of ethylene glycol in combination with sucrose are used and high cooling rates greater than the critical cooling rate for avoidance of ice formation are used in a method which results in vitrification of the tissue slices without toxicity and with increased viability after rewarming. Methods and materials can be used for production of PCLS which can be stored at cryoprotective temperatures for extended periods of time.