Non-toxigenic fungal strains, and methods of making and use thereof, are provided and have utility as endophytes in forage crops, and as strains that can outcompete toxigenic strains in forage and food crops.

The present invention relates to Pleurotus eryngii var. ferulae. More particularly, the present invention relates to a novel Pleurotus eryngii var. ferulae strain line following a line of DDL01 (KACC93085P) which is obtained by selecting a conventionally known Pleurotus eryngii var. ferulae strain and then cross-breeding the selected strain with the Pleurotus eryngii var. ferulae strain DDL01 (accession number: KACC93085P), and also relates to a fruit body obtained through cultivation of the strain.

The present invention relates to Pleurotus eryngii var. ferulae. More particularly, the present invention relates to a novel Pleurotus eryngii var. ferulae strain line following a line of DDL01 (KACC93085P) which is obtained by selecting a conventionally known Pleurotus eryngii var. ferulae strain and then cross-breeding the selected strain with the Pleurotus eryngii var. ferulae strain DDL01 (accession number: KACC93085P), and also relates to a fruit body obtained through cultivation of the strain.

A method of improving the viability, persistence, and/or vigor of a host plant in the form of Lolium multiflorum Lolium x hybridum and Lolium perenne, (host grass) includes artificially inoculating the host grass with an LpTG-3 endophyte RGT18 strain. The LpTG-3 endophyte RGT18 strain can produce at least one alkaloid or combination of alkaloids that provide the host grass with a favorable alkaloid profile and/or protect the plant from biotic stresses.

The invention describes a methodology for production of a secondary extra-particle fungal matrix for application as a mycological material, manufactured via a Type II actively aerated static packed-bed bioreactor. A pre-conditioned air stream is passed through a substrate of discrete elements inoculated with a filamentous fungus to form an isotropic inter-particle hyphal matrix between the discrete elements. Continued feeding of the air through the substrate of discrete elements and isotropic inter-particle hyphal matrixes develops an extra-particle hyphal matrix that extends from an isotropic inter-particle hyphal matrix in the direction of airflow into a void space within the vessel.

The invention describes a methodology for production of a secondary extra-particle fungal matrix for application as a mycological material, manufactured via a Type II actively aerated static packed-bed bioreactor. A pre-conditioned air stream is passed through a substrate of discrete elements inoculated with a filamentous fungus to form an isotropic inter-particle hyphal matrix between the discrete elements. Continued feeding of the air through the substrate of discrete elements and isotropic inter-particle hyphal matrixes develops an extra-particle hyphal matrix that extends from an isotropic inter-particle hyphal matrix in the direction of airflow into a void space within the vessel.

The present invention relates to the development of a homokaryotic Agaricus bisporus (Lange) Imbach mushroom fungus line culture designated N-s34 and to cultures obtained, descended, or otherwise derived from line N-s34. More particularly, the present invention relates to cultures incorporating at least one set of chromosomes having a genotype present in the genotype of the chromosomes found in line N-s34. The present invention further relates to F1 hybrids, and to a particular F1 hybrid strain, designated LA3782, descended from N-s34. This particular strain indeed displays an excellent yield weight of the harvested crop, especially in the third-flush, and a very good shelf-life of the mushroom products. The invention additionally relates to progeny, lines and strains derived from or descended from, or otherwise developed or obtained from, line N-s34 and from said hybrid strain LA3782. The invention further relates to methods of use of the cultures described hereinabove.

The present invention relates to the development of a homokaryotic Agaricus bisporus (Lange) Imbach mushroom fungus line culture designated N-s34 and to cultures obtained, descended, or otherwise derived from line N-s34. More particularly, the present invention relates to cultures incorporating at least one set of chromosomes having a genotype present in the genotype of the chromosomes found in line N-s34. The present invention further relates to F1 hybrids, and to a particular F1 hybrid strain, designated LA3782, descended from N-s34. This particular strain indeed displays an excellent yield weight of the harvested crop, especially in the third-flush, and a very good shelf-life of the mushroom products. The invention additionally relates to progeny, lines and strains derived from or descended from, or otherwise developed or obtained from, line N-s34 and from said hybrid strain LA3782. The invention further relates to methods of use of the cultures described hereinabove.

The present invention relates to a mutant of a parent filamentous fungal cell, comprising: a polynucleotide encoding a polypeptide of interest; a racA gene encoding a Rho-GTPase RacA protein, wherein the racA gene is modified rendering the mutant partially or completely deficient in the production of the Rho-GTPase RacA protein; and a ras2 gene encoding a GTPase Ras2 protein, wherein the GTPase Ras2 protein is modified to produce a GTPase Ras2 variant comprising a substitution at a position corresponding to position 16 of SEQ ID NO: 11, wherein the combination of the modified racA gene and the Ras2 variant synergistically increases the productivity of the mutant in the production of the polypeptide of interest. The present invention also relates to a method of producing a polypeptide of interest with such a mutant.

A new variety of shiitake mushroom that has a medium density, late growth according to temperature, an overall scaled cap, an average length of time from spawning to fruiting, and high yield at second and third month.